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KMID : 0359320100500020079
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Korean Journal of Veterinary Research
2010 Volume.50 No. 2 p.79 ~ p.84
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Norepinephrine induces MAIL mRNA expression in primary cultured hepatocytes through IL-1β released from non-parenchymal cells
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Kim Hyeon-Cheol
Jung Bae-Dong
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Abstract
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The molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL) protein is a novel member of the Ikappaβ family. In the present study, we examined the effect of norepinephrine (NE) on MAIL mRNA expression in primary cultured mouse hepatocytes and non-parenchymal liver cells. MAIL mRNA expression in hepatocytes and non-parenchymal liver cells was not directly influenced by NE. However, MAIL mRNA expression in hepatocytes was significantly induced by incubation with a culture medium of non-parenchymal liver cells, treated with NE. Pretreatment with an interleukin (IL)-1 receptor antagonist significantly attenuated the stimulatory effect of the medium. Moreover, exogenous IL-1β induced MAIL mRNA expression in hepatocytes, while IL-6 and tumor necrosis factor α did not. The concentration of IL-1β in the medium of non-parenchymal liver cells was significantly increased after NE-treatment. These results suggest that NE can induce MAIL mRNA expression in hepatocytes through IL-1β, released from non-parenchymal liver cells.
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KEYWORD
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hepatocytes, interleukin-1, MAIL, non-parenchymal liver cells, norepinephrine
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